Publications

@article{Martínez2025,

title = {Enzymatic activity responses to transport and low-temperature storage: implication for plant nitrogen metabolism studies},

author = {D. N. Martínez and E. de la Barrera},

url = {https://www.mdpi.com/2504-3129/6/1/5

https://agro.mx/wp-content/uploads/2025/01/077-Martinez-N-cold-enzymes.pdf},

doi = {10.3390/nitrogen6010005},

isbn = {2504-3129},

year  = {2025},

date = {2025-01-16},

urldate = {2025-01-16},

journal = {Nitrogen},

volume = {6},

abstract = {Understanding how transport and storage conditions affect enzymatic activity is essential for accurate biomonitoring of nitrogen metabolism in plants. This study evaluated the effects of transport conditions and low-temperature storage on the enzymatic activities of nitrate reductase (NR), glutamine synthetase (GS), and phosphomonoesterase (PME) for Chloris gayana, Fraxinus uhdei, and Trifolium repens. Enzymatic activities were measured for leaf samples immediately after collection, after 18 h at room temperature, or after 18 h on ice. Additionally, samples were stored at –16°C or –45°C for up to 28 days. NR activity decreased to near-zero levels under all storage conditions, indicating that this enzyme is unsuitable for delayed analysis. In contrast, GS and PME activities showed species-dependent responses to storage, with increased activity ob-served for T. repens and C. gayana, potentially reflecting tissue degradation processes. F. uhdei exhibited greater stability in enzyme activities, suggesting a higher resilience to storage. These findings highlight the importance of minimizing storage time to preserve enzymatic integrity, particularly for NR, while providing insight into the potential for delayed analysis of GS and PME in specific species. This work offers practical recommendations for future biomonitoring ef-forts in nitrogen deposition studies.},

keywords = {disturbance, neotropical, nitrate reductase, nitrogen deposition, phosphorus, planetary boundaries, plant nutrition, tropical forest, urban ecology},

pubstate = {published},

tppubtype = {article}

}

@article{Osuna-Vallejo2019,

title = {Total mercury in plant tissue from a mining landscape in Western Mexico},

author = {V. Osuna-Vallejo and C. Sáenz-Romero and L. Escalera-Vázquez and E. de la Barrera and R. Lindig-Cisneros},

doi = {10.1007/s00128-018-2488-0},

year  = {2019},

date = {2019-01-15},

journal = {Bulletin of Environmental Contamination & Toxicology},

volume = {102},

number = {1},

pages = {19-24},

abstract = {Environmental impacts of mining activities are well known, particularly on-site degradation, but long term effects are less known. Mercury content from vegetation samples from a mine dump and surrounding forests was quantified for understanding the fate of this element in the local the environment. The study area, Tlalpujahua, Michoacán, México, has a mining history going back more than 400 years. Including gold and silver extraction by means of mercury amalgamation for 352 years (1554–1906). Mercury was present in all sampled materials. The highest values correspond to wood samples from the mine dump (13.84 ± 3.88 ppm), while wood samples from adjacent forests had 4.3 ± 2.4 ppm, almost twice as much as coniferous needles, shrub leaves and corn seeds (2.2 ± 0.34 ppm). The highest concentration was found for J. deppeana wood (16.05 ± 2.3 ppm). The capacity of accumulating mercury by Juniperus trees when growing on the mine dumps suggests that this species has a potential to be used for biosequestration purposes.},

keywords = {agriculture, biomonitors, conifers, pollution, temperate forest, tropical forest},

pubstate = {published},

tppubtype = {article}

}

@article{delaBarrera2005b,

title = {Challenges to plant megadiversity: how environmental physiology can help},

author = {E. de la Barrera and J. L. Andrade},

url = {https://agro.mx/wp-content/uploads/2016/06/012-De-la-Barrera-Andrade-2005-New-Phytol.pdf},

year  = {2005},

date = {2005-05-01},

journal = {New Phytologist},

volume = {167},

pages = {5-8},

keywords = {acclimation, agriculture, cactus, climate change, conservation, ecophysiology, legume, nitrogen, plant diversity, public policy, seed, stable isotopes, tropical forest},

pubstate = {published},

tppubtype = {article}

}

@article{Nobel2004,

title = {CO2 uptake by the cultivated hemiepiphytic cactus, Hylocereus undatus},

author = {P. S. Nobel and E. de la Barrera},

url = {https://agro.mx/wp-content/uploads/2015/09/010-Nobel-De-la-Barrera-2004-Ann-Appl-Biol.pdf},

year  = {2004},

date = {2004-04-04},

journal = {Annals of Applied Biology},

volume = {144},

pages = {1-8},

keywords = {abscisic acid, agriculture, cactus, CO2, cultivation, Hylocereus, nitrogen, photosynthesis, pitahaya, temperature, tropical forest, water},

pubstate = {published},

tppubtype = {article}

}

@article{Nobel2002,

title = {High temperature and net CO2 uptake, growth, and stem damage for the hemiepiphytic cactus Hylocereus undatus},

author = {P. S. Nobel and E. de la Barrera},

url = {https://agro.mx/wp-content/uploads/2016/06/002-Nobel-De-la-Barrera-2002-Biotropica.pdf},

year  = {2002},

date = {2002-02-01},

journal = {Biotropica},

volume = {34},

number = {2},

pages = {225-231},

keywords = {acclimation, cactus, CAM, desert, fruit, Hylocereus, necrosis, neutral red, pitahaya, root, temperature, tropical forest},

pubstate = {published},

tppubtype = {article}

}