Publications

@article{Martínez2025,

title = {Enzymatic activity responses to transport and low-temperature storage: implication for plant nitrogen metabolism studies},

author = {D. N. Martínez and E. de la Barrera},

url = {https://www.mdpi.com/2504-3129/6/1/5

https://agro.mx/wp-content/uploads/2025/01/077-Martinez-N-cold-enzymes.pdf},

doi = {10.3390/nitrogen6010005},

isbn = {2504-3129},

year  = {2025},

date = {2025-01-16},

urldate = {2025-01-16},

journal = {Nitrogen},

volume = {6},

abstract = {Understanding how transport and storage conditions affect enzymatic activity is essential for accurate biomonitoring of nitrogen metabolism in plants. This study evaluated the effects of transport conditions and low-temperature storage on the enzymatic activities of nitrate reductase (NR), glutamine synthetase (GS), and phosphomonoesterase (PME) for Chloris gayana, Fraxinus uhdei, and Trifolium repens. Enzymatic activities were measured for leaf samples immediately after collection, after 18 h at room temperature, or after 18 h on ice. Additionally, samples were stored at –16°C or –45°C for up to 28 days. NR activity decreased to near-zero levels under all storage conditions, indicating that this enzyme is unsuitable for delayed analysis. In contrast, GS and PME activities showed species-dependent responses to storage, with increased activity ob-served for T. repens and C. gayana, potentially reflecting tissue degradation processes. F. uhdei exhibited greater stability in enzyme activities, suggesting a higher resilience to storage. These findings highlight the importance of minimizing storage time to preserve enzymatic integrity, particularly for NR, while providing insight into the potential for delayed analysis of GS and PME in specific species. This work offers practical recommendations for future biomonitoring ef-forts in nitrogen deposition studies.},

keywords = {disturbance, neotropical, nitrate reductase, nitrogen deposition, phosphorus, planetary boundaries, plant nutrition, tropical forest, urban ecology},

pubstate = {published},

tppubtype = {article}

}

@article{Alcántara-Plazola2021,

title = {Quantification of embedded phosphorus in Mexican agriculture},

author = {J. J. Alcántara-Plazola and E. de la Barrera},

url = {https://www.sciencedirect.com/science/article/abs/pii/S2352550921002104},

doi = {10.1016/j.spc.2021.07.011},

issn = {2352-5509},

year  = {2021},

date = {2021-07-14},

journal = {Sustainable Production and Consumption},

volume = {28C},

pages = {824-828},

abstract = {Phosphorus is an essential element for agricultural production, and for life in general, whose availability as a mineral is geographically restricted. Considering that Mexico is a major contributor to the global flow of water embedded in agricultural commodities, it is likely that this country is also a major exporter of embedded phosphorus. Thus, we quantified the embedded phosphorus for the 38 major crops produced in Mexico from 1980 to 2015, which were grouped into forages, fruits and vegetables, cereals, legumes, and cash crops. The total phosphorus extracted was 4.4 × 106 tonnes for an agricultural production of 5,844 × 106 tonnes, which respectively increased by 86% and 145% over 35 years. Food crops, i.e., cereals, fruits and vegetables, and legumes, accounted for 44% of the total phosphorus extracted, forages for 38%, and cash crops for 18%. Forage production exhibited a substantial increase since 1995, becoming the largest phosphorus extractor by 2000. Our estimation of embedded phosphorus is susceptible of utilization in other countries and is a first step to estimate a country's phosphorus balance, which is necessary to understand the magnitude of the anthropogenic alteration of a biogeochemical cycle that is essential for the maintenance of life on earth.},

keywords = {agriculture, embedded phosphorus, food security, phosphorus, planetary boundaries, telecoupling},

pubstate = {published},

tppubtype = {article}

}

@article{Hernández2020,

title = {Cooperation between Sporobolus airodes and associated arbuscular mycorrhizal fungi for phosphorus acquisition under drought conditions in an oligotrophic desert ecosystem},

author = {D. Hernández-y-Hernández and J. Larsen and A. González-Rodríguez and Y. Tapia-Torres and E. de la Barrera and L.E. Eguiarte and F. García-Oliva},

doi = {10.1016/j.rhisph.2020.100225},

year  = {2020},

date = {2020-07-04},

journal = {Rhizosphere},

volume = {15},

number = {100225},

pages = {1-11},

abstract = {Plants from phosphorus (P) limited environments have developed various strategies for acquiring P from the soil , and for maintaining P homeostasis within the plant. We analyzed the mechanisms of phosphorus acquisition used by the grass Sporobolus airoides -rhizosphere microbiota during two years with contrasting annual rainfall in an extreme P-oligotrophic ecosystem. We determined arbuscular mycorrhizal fungi (AMF) root and soil colonization , and the activity of three P-related eco-enzymes over two consecutive years. We also determined C, N, and P concentrations and ratios in plant biomass, microbial biomass and soil for assessing nutrient homeostasis of soil microbial community and plants. S. airoides invested more carbon in the AMF symbiosis in the drought year in the site more limited by P (Pozas Azules site). Additionally, the specific activity of phosphomonoesterases was higher in this site, suggesting that the microbial community invests more energy to produce this enzyme to release P from organic molecules. In conclusion, our results show evidence of the importance of AMF symbiosis for S. airoides to cope with P and water limitations in a highly oligotrophic ecosystem.},

keywords = {arid, desert, mycorrhiza, nutrients, phosphorus},

pubstate = {published},

tppubtype = {article}

}

@article{Gómez-Romero2013,

title = {Fertilización y asociación con especies pioneras herbáceas en el crecimiento de Pinus pseudostrobus},

author = {M. Gómez-Romero and E. de la Barrera and J. Villegas and R. Lindig-Cisneros},

url = {http://www.revistaphyton.fund-romuloraggio.org.ar/vol82/GOMEZ_ROMERO.pdf},

year  = {2013},

date = {2013-12-12},

journal = {Phyton},

volume = {82},

pages = {135-143},

keywords = {erosion, Lupinus, nitrogen, phosphorus, Pinus, pioneer, restoration ecology, temperate forests, understory},

pubstate = {published},

tppubtype = {article}

}

@article{Escutia-Lara2010,

title = {Respuesta a la adición de nitrógeno y fósforo en el crecimiento de Typha domingensis y Schoenoplectus americanus},

author = {Y. Escutia-Lara and E. de la Barrera and Y. Martínez-delaCruz and R. Lindig-Cisneros},

url = {http://www.scielo.org.mx/scielo.php?pid=S0366-21282010000200007&script=sci_arttext},

year  = {2010},

date = {2010-01-01},

journal = {Boletín de la Sociedad Botánica de México},

volume = {87},

pages = {83-87},

keywords = {nitrogen, nutrition, phosphorus, Schoenoplectus, Typha, wetland},

pubstate = {published},

tppubtype = {article}

}